abstract：:Previous work has shown that purified rat macrophages lack both accessory activity for T lymphocyte responses to mitogens and stimulatory activity in a mixed leukocyte reaction, in marked contrast to the potent activity of dendritic cells. This study was designed to re-evaluate macrophages as accessory cells by treati...

journal_title：Immunobiology

authors： Bowers WE,Ruhoff MS,Goodell EM,Stoltenborg JK

更新日期：1988-02-01 00:00:00

abstract：:BALB/c mice injected with 0.2 mg TNP-ovalbumin (OA) within 24 hours after birth showed reduced levels of functionally active TNP-specific B cells and, accordingly, of plaque-forming cells (PFC) after challenge with carrier-bound TNP until the age of 8 weeks. Yet, when B cells of tolerized mice were cultured in the abs...

journal_title：Immunobiology

authors： Zöller M,Andrighetto G

更新日期：1987-12-01 00:00:00

abstract：:The course of legionella pneumonia in guinea pigs after infection with various quantities of virulent L. pneumophila serogroup 1 organisms by aerosol exposure was investigated. The clinical course, histopathological characteristics, manifestations in the lungs and clearance of the legionella organisms from the lungs a...

journal_title：Immunobiology

authors： Twisk-Meijssen MJ,Meenhorst PL,van Cronenburg BJ,Mulder JD,Scheffer E,van Furth R

更新日期：1987-12-01 00:00:00

abstract：:In a rat model, we compared the effects of various soluble products released by T cells, such as the unspecifically acting gamma-interferon or the newly detected alloantigen-induced factors that specifically act on nonlymphoid, hemopoietic bone marrow cells. We found two types of reactivity patterns with regard to the...

journal_title：Immunobiology

authors： Wottge HU,Müller-Ruchholtz W

更新日期：1987-12-01 00:00:00

abstract：:Recently, it could be shown that Cyclosporin A (CsA) inhibited the activation of T and B lymphocytes by interfering with an early step of the activation, namely the stimulation of the plasma membrane-bound lysophosphatide acyltransferase. In this report, we compared three CsA-derivatives, Dihydro-Cyclosporin C (Dihydr...

journal_title：Immunobiology

authors： Kroggel R,Goppelt-Strübe M,Martin M,Resch K

更新日期：1987-09-01 00:00:00

abstract：:By fusion of C3H/HeJ splenic adherent mononuclear cells enriched for macrophages with HPRT-deficient C57L/J HH- hepatoma cells, we have generated six macrophage-hepatoma hybrid clones. The hybrid nature of isolated clones was demonstrated by karyotypic analysis. The hybrid clones were screened for macrophage propertie...

journal_title：Immunobiology

authors： Chou YK,Stanley W,Virella G,Kilpatrick JM,Fudenberg HH

更新日期：1987-08-01 00:00:00

abstract：:The suppressive activity of two bovine serum albumin-specific class II-restricted T suppressor cell clones (BVI/5 and 83/2-D11) was compared to that of a feeder cell-independent, IL 2-dependent subline (HF1.IL-2) of an originally antigen-dependent class II-restricted Ts cell clone (HF1). No soluble suppressor factors ...

journal_title：Immunobiology

authors： Bettmann R,Degwert J,Heuer J,Kölsch E

更新日期：1987-05-01 00:00:00

abstract：:In an immunohistochemical study using monoclonal antibodies, which exclusively recognize cells of the monocyte-macrophage lineage, and monoclonal antibodies against the Ia-antigen, we describe the occurrence of macrophages in the developing and adult central nervous system (CNS). In normal adult brain, no macrophages ...

journal_title：Immunobiology

authors： Sminia T,de Groot CJ,Dijkstra CD,Koetsier JC,Polman CH

更新日期：1987-01-01 00:00:00

abstract：:Human recombinant erythropoietin (rHuEPO) has been purified to apparent homogeneity and compared to purified human urinary erythropoietin (EPO). Both the purified natural and recombinant EPO preparations were characterized in a competition radioimmunoassay (RIA), the exhypoxic polycythemic mouse bioassay, in vitro tis...

journal_title：Immunobiology

authors： Egrie JC,Strickland TW,Lane J,Aoki K,Cohen AM,Smalling R,Trail G,Lin FK,Browne JK,Hines DK

更新日期：1986-09-01 00:00:00

abstract：:Fifty-nine patients with metastatic malignant melanoma were entered into a phase II trial of recombinant alpha-2 interferon given in a dosage of 10 million IU/M2 subcutaneously three times per week for one year. Forty-five of these were evaluable for response. Of five evaluable patients with ocular primaries, none res...

journal_title：Immunobiology

authors： Robinson WA,Mughal TI,Thomas MR,Johnson M,Spiegel RJ

更新日期：1986-09-01 00:00:00

abstract：:Human urinary colony-stimulating factor (CSF-HU) has been partially purified using five purification procedures (preparation II) for clinical use. In phase I study, six healthy volunteers were injected with preparation II of CSF-HU by a single intravenous droplet infusion. Two volunteers received 4 X 10(6) units (U), ...

journal_title：Immunobiology

authors： Motoyoshi K,Ishizaka Y,Miura Y,Takaku F

更新日期：1986-09-01 00:00:00

abstract：:Tumor necrosis factor (TNF) has been determined as an endogenous mediator for endotoxin-induced tumor necrosis. This macrophage product has been biochemically characterized and its protein structure defined by molecular cloning of the TNF gene. Experiments with antibodies to TNF demonstrated that TNF acts as an effect...

journal_title：Immunobiology

authors： Männel DN

更新日期：1986-09-01 00:00:00

abstract：:The activation of cytotoxic T lymphocytes (CTL) in vivo was found to be augmented by glutathione if injected i.p. in the late phase but not in the early phase of the response. The effect of glutathione possibly resembles the augmenting effect of 2-mercaptoethanol in lymphocyte cultures. ...

journal_title：Immunobiology

authors： Dröge W,Pottmeyer-Gerber C,Schmidt H,Nick S

更新日期：1986-08-01 00:00:00

abstract：:The macrophage population of the gastro-intestinal tract of the rat was studied by using a set of three monoclonal antibodies (ED1, ED2 and ED3) directed against rat macrophages, an anti-Ia monoclonal antibody and by performing an acid phosphatase reaction. The lamina propria of the whole gastro-intestinal tract compr...

journal_title：Immunobiology

authors： Sminia T,Jeurissen SH

更新日期：1986-08-01 00:00:00

abstract：:Cell walls from Streptococcus pyogenes strain Sv (Group A, M type 3) were lysed with M1 endo-N-acetylmuramidase, and the group A-specific carbohydrate antigen was purified by Sephadex G-100 gel filtration. The initial eluting antigen (M1gA) peak was assessed for mitogenic and polyclonal lymphocyte-activating propertie...

journal_title：Immunobiology

authors： Morisaki I,Kimura S,Torii M,Michalek SM,McGhee JR,Okahashi N,Hamada S

更新日期：1985-11-01 00:00:00

abstract：:The specificity of cytotoxic T lymphocyte (CTL) responses generated in the presence of lymphokines was studied. Thymic responder cells were activated in the presence of stimulator cells that differed in their metabolic activity. After 5 days of culture, the cytotoxic response was estimated in a 4-h 51Cr-release test. ...

journal_title：Immunobiology

authors： Männel DN,Dröge W,Falk W

更新日期：1985-09-01 00:00:00

abstract：:The anticonvulsant drug phenytoin, in less than cytotoxic concentrations, caused significant reductions in Ig secretion by unstimulated or EBV-stimulated normal MNC, as measured by PFC or secretion of Ig into the culture medium. Isotype-specific LBL varied in their sensitivity, the secretion of IgA (1 line) and IgG (3...

journal_title：Immunobiology

authors： Wangel AG,Arvilommi H,Jokinen I

更新日期：1985-09-01 00:00:00

abstract：:We investigated the effects of ML I and its isolated chains, A and B, in regard to selected functions of phagocytes (human granulocytes, paraffin-oil stimulated M phi from guinea pigs). On these cells, ML I has no cytotoxic effect between 10(-14) and 10(-8) (trypan blue exclusion and ethidium bromide exclusion). Over ...

journal_title：Immunobiology

authors： Metzner G,Franz H,Kindt A,Fahlbusch B,Süss J

更新日期：1985-07-01 00:00:00

abstract：:Intravenous (i.v.) immunization of mice with irradiated (2000 rads) allogeneic lymphoid cells induces the generation of suppressor T cells. Such suppressor T cells can suppress the anti-host delayed type hypersensitivity (DTH) by other T cells during acute Graft-versus-Host reactions in irradiated recipient mice. Supp...

journal_title：Immunobiology

authors： Bril H,Molendijk-Lok BD,Hussaarts-Odijk LM,Benner R

更新日期：1985-04-01 00:00:00

abstract：:Activity of acid phosphatase was found to be higher in T than in non-T cells of human peripheral blood lymphocytes. The T cell mitogen PHA induced an increase in the magnitude of acid phosphatase activity, while the B cell mitogen LPS was not able to produce any effect on acid phosphatase activity. Acid phosphatase ac...

journal_title：Immunobiology

authors： Myśliwska J,Myśliwski A,Grzegorzewski K,Witkowski J

更新日期：1985-03-01 00:00:00

abstract：:Using irradiation bone marrow chimeras to analyze restriction specificity of helper T cells, we found that recipient H-2 type dictated the H-2 type which the T cells recognize as self (adaptive differentiation). T cells from (H-2b----H-2k) chimeras cooperate with non-T cells bearing Iak to generate a vigorous PFC resp...

journal_title：Immunobiology

authors： Onoé K,Yasumizu R,Noguchi M,Iwabuchi K,Ogasawara M,Kakinuma M,Okuyama H,Good RA,Morikawa K

更新日期：1985-02-01 00:00:00

abstract：:A mouse ear reaction for testing cell mediated immunity of human lymphocytes using the local graft-versus-host reaction assay is described. Five million human mononuclears were locally injected into the ears of immune suppressed NZW mice. The reaction mounted was quantitated by determining the 125I-Iodo-Deoxyuridine (...

journal_title：Immunobiology

authors： Mukamel E,Umiel T,Shohat B,Pecht M,Servadio C,Trainin N

更新日期：1985-02-01 00:00:00

abstract：:The part played by MHC antigens on lymphocyte homing has been investigated by inducing modifications of their expression on lymphoid cells. A significant, though moderate, enhancement of H-2 antigen expression has been observed following in vitro treatment of lymphocytes with alpha-IFN. A decrease has been obtained af...

journal_title：Immunobiology

authors： Navarro J,El Ansary M,Royo C,de Maeyer E,Touraine JL

更新日期：1984-12-01 00:00:00

abstract：:The recent purification and cloning of T cell-derived growth factors, should lead to rapid progress in delineating their role in the generation of accessory cells and the regulation of their function. The techniques that have allowed detailed in vitro studies of the T-dependent mast cells, should be generally applicab...

journal_title：Immunobiology

authors： Schrader JW,Clark-Lewis I,Wong GH

更新日期：1984-12-01 00:00:00

abstract：:The T effector lymphocytes of delayed type hypersensitivity reactions (TDH) are regulated by a complex T suppressor (Ts) cell circuit. Induction of TDH cells requires Ia+ adherent cells as antigen-presenting cells. Little is known about the antigen presentation of the induction of Ts cells. We describe an experimental...

journal_title：Immunobiology

authors： Knop J,Malorny U,Michels E,Sorg C

更新日期：1984-12-01 00:00:00

abstract：:Exposure of resident peritoneal macrophages or thioglycollate-induced macrophages (TG-Mø) to influenza or Sendai virus-infected spleen cell culture supernatants (MAS) resulted in macrophage activation. When normal resident macrophages were used as effector cells, both infected P815 and L929 cells were lysed in the pre...

journal_title：Immunobiology

authors： Mak NK,Ada GL

更新日期：1984-05-01 00:00:00

abstract：:An ELISA with covalently fixed antigens was used to measure and define ferritin-labeled antibodies. In the presented model, RIG covalently fixed to glass tubes served as antigen. Twenty ferritin-labeled SWaRIG conjugates were prepared with different molar ratios of antibody: ferritin: glutaraldehyde. At various diluti...

journal_title：Immunobiology

authors： Rauterberg EW,Schieck C,Kreft H,Römer W

更新日期：1984-05-01 00:00:00

abstract：:We were able to characterize about 25 antigenic proteins of cultured synovial fibroblasts with apparent molecular weights of 15-230 kd by SDS-electrophoresis and immunoblotting using guinea pig antiserum. Some of these proteins were bovine-serum-derived, adsorbed onto fibroblasts from culture medium. Both rheumatoid a...

journal_title：Immunobiology

authors： Heino J,Kouri T,Jalkanen M,Lehtonen OP

更新日期：1984-05-01 00:00:00

abstract：:Culture supernatants (CS) from Hodgkin derived cell lines have previously been shown to contain colony stimulating activity (CSF) for human cord blood cells, fetal bone marrow and fetal liver cells. In this study 3-day CS from four Hodgkin lines (L428, L538, L540, L591) and two sublines (L428KS, L428KSA) were examined...

journal_title：Immunobiology

authors： Kortmann C,Burrichter H,Monner D,Jahn G,Diehl V,Peter HH

更新日期：1984-05-01 00:00:00

abstract：:The polymorphonuclear neutrophil (PMN) regulates in vivo and in vitro immune responses. We report that the immunoenhancing properties of PMN culture supernatants from PMN recruited by the bacterium Actinomyces viscosus (AV) show its exclusive effects on the T cell lymphocyte population. A study of the effect of PMN su...

journal_title：Immunobiology

authors： Fitzgerald J,West K,Sonis S,Wilson R

更新日期：1983-12-01 00:00:00

abstract：:A glycoprotein from human erythrocyte membranes has been found to inhibit lysis of target cells by the attack-phase components C5-C9 from human complement. The inhibiting molecule was purified and identified as glycophorin A. Thus, glycophorin A may have a regulatory function in the lytic complement attack on isologou...

journal_title：Immunobiology

authors： Brauch H,Roelcke D,Rother U

更新日期：1983-08-01 00:00:00

abstract：:In this study, the effect of sixteen different enzymes on serum C1 and its subcomponents was investigated. The sixteen enzymes could be divided into three groups. First, enzymes which activate native C1: trypsin (optimal concentration 2.4 x 10(-4) mM); alpha-chymotrypsin (2.3 x 10(3) mM); thrombin (1.0 x 10(-5) mM); p...

journal_title：Immunobiology

authors： Heinz HP,Loos M

更新日期：1983-08-01 00:00:00

abstract：:This study demonstrates immune complexes in thyroid glands of Obese strain (OS) chickens, that consist of thyroglobulin (Tg) and antibodies to Tg. In IIF tests it was shown that these complexes fix complement with an age-dependent increase from 12% in 19-day-old embryos up to 100% in 6-week-old animals. This finding a...

journal_title：Immunobiology

authors： Kofler H,Kofler R,Wolf H,Wick G

更新日期：1983-05-01 00:00:00

abstract：:A modified immunoperoxidase assay on microscope slides (IPSA) was adopted as a screening procedure for hybridoma supernatants with specificity for human lymphocyte subpopulations. The method proved to be suitable for testing a large number of hybridomas with a minimum of cells and reagents. In addition, the slide-tech...

journal_title：Immunobiology

authors： Morich FJ,Momburg F,Moldenhauer G,Hartmann KU,Bross KJ

更新日期：1983-03-01 00:00:00

abstract：:Erythrocytes from paroxysmal nocturnal hemoglobinuria patients (PNH-E) are much more susceptible to lysis by acid-activated human serum than normal human erythrocytes. Acidification of normal human serum to pH 6.4 in the absence of erythrocytes generates this lytic activity independently of the alternative pathway of ...

journal_title：Immunobiology

authors： Hänsch G,Hammer C,Jiji R,Rother U,Shin M

更新日期：1983-03-01 00:00:00

abstract：:The aim of the work was to justify the use of trifluoromethyl-dinitrobenzene sulfonate (CF3-DNBS) modification rather than trinitrobenzene-sulfonate (TNBS) modification, so as to be able to take advantage of the presence of fluorine atoms in the analogue, which allow the analysis of the hapten-carrier bonds by 19F-NMR...

journal_title：Immunobiology

authors： Bischoff P,Maugras M,Llorens MC,Oth D

更新日期：1983-02-01 00:00:00

abstract：:Peritoneal as well as peripheral blood, but not spleen macrophages inhibit natural killer (NK) cell activity directed against adherent growing target cells. Inhibition was observed with macrophages (Mø): spleen cell ratios as low as 0.01:1. It was independent from the activation status of Mø and was not MHC restricted...

journal_title：Immunobiology

authors： Zöller M,Matzku S

更新日期：1982-12-01 00:00:00

abstract：:The inability of spontaneous and some laboratory-induced tumors to stimulate the immune system has continuously raised the question of the validity of using immunological maneuvers in order to control tumor growth. In this project we suggest that a tumor which is nonimmunogenic still has an immunogenic potential that ...

journal_title：Immunobiology

authors： Klein BY,Sharon R,Tarcic N,Naor D

更新日期：1982-10-01 00:00:00

abstract：:When sheep red blood cells (SRBC) were injected intraperitoneally (i.p.), intravenously (i.v.), or into the Peyer's patches, definite plaque-forming cell (PFC) and rosette-forming cell (RFC) responses were induced in the spleen but not in the mesenteric lymph nodes (MLN). When SRBC were injected directly into the sple...

journal_title：Immunobiology

authors： Kojima T,Kamei H,Kondo T,Nakashima I,Kato N

更新日期：1982-05-01 00:00:00

abstract：:The ability of RPMI 1640 enriched with FCS or AATSZ (L-alanine, BSA, human transferrin, zinc chloride, and sodium selenite) to support mitogen-induced activation (G0-G1 shift) and proliferation (G1-S shift) of thymocytes has been investigated. The two culture media were found to be equally supportive. In terms of viab...

journal_title：Immunobiology

authors： Kristensen F,Walker C,Müller J,de Weck AL

更新日期：1982-05-01 00:00:00